Assay of urinary free and conjugated 3-methoxy-4-hydroxyphenyleneglycol by high-performance liquid chromatography with amperometric detection
Identifieur interne : 001628 ( France/Analysis ); précédent : 001627; suivant : 001629Assay of urinary free and conjugated 3-methoxy-4-hydroxyphenyleneglycol by high-performance liquid chromatography with amperometric detection
Auteurs : R. Bourdeaux [France] ; B. Capolaghi [France] ; C. Lacroix [France] ; C. Younos [France] ; P. R. Lehr [France]Source :
- Journal of Chromatography B: Biomedical Sciences and Applications [ 0378-4347 ] ; 1985.
Abstract
The aim of this study was to develop an analytical method for free and conjugated 3-methoxy-4-hydroxy-phenylethyleneglycol (MHPG) in urine. After hydrolysis of the conjugated forms, the urinary MHPG was purified by solid-phase extraction on anion exchanger and eluted with a water-methanol (1:1, v/v) mixture. After addition of ethyl acetate to the eluate and back-extraction into acetic acid, the aqueous phase was separated on a C18 column by HPLC and detected amperometrically. The results obtained from forty healthy human subjects were compared with the literature values. The precision and accuracy of the assay were studied using 4-methoxy-3-hydroxyphenylethyleneglycol (iso-MHPG) as internal standard.
Url:
DOI: 10.1016/0378-4347(94)00516-8
Affiliations:
- France
- Grand Est, Haute-Normandie, Lorraine (région), Région Normandie
- Le Havre, Metz, Nancy, Thionville
- Université Paul Verlaine - Metz
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Younos</name><affiliation wicri:level="4"><country xml:lang="fr">France</country><wicri:regionArea>Centre des Sciences de l'Environnement, Université de Metz, 57000 Metz</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region><settlement type="city">Metz</settlement></placeName><orgName type="university">Université Paul Verlaine - Metz</orgName></affiliation></author><author><name sortKey="Lehr, P R" sort="Lehr, P R" uniqKey="Lehr P" first="P. R." last="Lehr">P. R. Lehr</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Biologie et Physiologie du Comportement, URA CNRS 1293, Faculté des Sciences, Université de Nancy I, 54000 Nancy</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region><settlement type="city">Nancy</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Chromatography B: Biomedical Sciences and Applications</title><title level="j" type="abbrev">CHROLD</title><idno type="ISSN">0378-4347</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1985">1985</date><biblScope unit="volume">665</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="45">45</biblScope><biblScope unit="page" to="51">51</biblScope></imprint><idno type="ISSN">0378-4347</idno></series><idno type="istex">0894983E52D645B55CED9077E770DFE4286420F9</idno><idno type="DOI">10.1016/0378-4347(94)00516-8</idno><idno type="PII">0378-4347(94)00516-8</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0378-4347</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The aim of this study was to develop an analytical method for free and conjugated 3-methoxy-4-hydroxy-phenylethyleneglycol (MHPG) in urine. After hydrolysis of the conjugated forms, the urinary MHPG was purified by solid-phase extraction on anion exchanger and eluted with a water-methanol (1:1, v/v) mixture. After addition of ethyl acetate to the eluate and back-extraction into acetic acid, the aqueous phase was separated on a C18 column by HPLC and detected amperometrically. The results obtained from forty healthy human subjects were compared with the literature values. The precision and accuracy of the assay were studied using 4-methoxy-3-hydroxyphenylethyleneglycol (iso-MHPG) as internal standard.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Grand Est</li><li>Haute-Normandie</li><li>Lorraine (région)</li><li>Région Normandie</li></region><settlement><li>Le Havre</li><li>Metz</li><li>Nancy</li><li>Thionville</li></settlement><orgName><li>Université Paul Verlaine - Metz</li></orgName></list><tree><country name="France"><region name="Grand Est"><name sortKey="Bourdeaux, R" sort="Bourdeaux, R" uniqKey="Bourdeaux R" first="R." last="Bourdeaux">R. Bourdeaux</name></region><name sortKey="Capolaghi, B" sort="Capolaghi, B" uniqKey="Capolaghi B" first="B." last="Capolaghi">B. Capolaghi</name><name sortKey="Capolaghi, B" sort="Capolaghi, B" uniqKey="Capolaghi B" first="B." last="Capolaghi">B. Capolaghi</name><name sortKey="Lacroix, C" sort="Lacroix, C" uniqKey="Lacroix C" first="C." last="Lacroix">C. Lacroix</name><name sortKey="Lehr, P R" sort="Lehr, P R" uniqKey="Lehr P" first="P. R." last="Lehr">P. R. Lehr</name><name sortKey="Younos, C" sort="Younos, C" uniqKey="Younos C" first="C." last="Younos">C. Younos</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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